Development of sensitive, high-throughput one-tube RT-PCR-enzyme hybridisation assay to detect selected bacterial fish pathogens
نویسندگان
چکیده
منابع مشابه
Development of a rapid, sensitive and specific diagnostic assay for fish Aquareovirus based on RT-PCR.
A rapid, sensitive and highly specific detection method for Aquareovirus based on reverse-transcription polymerase chain reaction (RT-PCR) was developed. Based on multiple sequence alignment of the cloned sequences of a local isolates, the Threadfin reovirus (TFV) and Guppy reovirus (GPV) with Grass carp reovirus (GCRV), a pair of degenerate primers was selected carefully and synthesized. Using...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2003
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao054127